We are aware that PCL can overlap with some variants of DAPI and Phalloidin stainings. As such we would recommend the following for cell visualisation:
1) Any DAPI variant which has an excitation wavelength close to 358 nm and emission wavelength close to 461 nm (e.g. Invitrogen D3571)
2) Any Phalloidin variant which has an excitation wavelength close to 495 nm and emission wavelength close to 513 nm. Phalloidin should be FITC-labelled.
We also provide validated DAPI/Phalloidin SOPs for our meshes as standard.